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Ubiquitin B 抗体

Rockland 200-401-431 UBB 适用: Various Species WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN108010
Supplier Product No.: 200-401-431
发货至: 中国
  • 抗原 See all Ubiquitin B (UBB) 抗体
    Ubiquitin B (UBB)
    适用
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    Various Species
    宿主
    • 38
    • 8
    克隆类型
    • 32
    • 14
    多克隆
    标记
    • 29
    • 3
    • 2
    • 2
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    This Ubiquitin B antibody is un-conjugated
    应用范围
    • 25
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    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Supplier Product No.
    200-401-431
    Supplier
    Rockland
    原理
    Ubiquitin Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
    产品特性
    Synonyms: rabbit anti-Ubiquitin Antibody, FLJ25987 antibody, MGC8385 antibody, Polyubiquitin B antibody, RPS27A antibody, UBA52 antibody, UBA80 antibody, UBB antibody, UBC antibody, UBCEP1 antibody
    纯化方法
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with ubiquitin coupled to rabbit IgG using glutaraldehyde.

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product UBB Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: This purified polyclonal antibody reacts with ubiquitin by ELISA and western blot. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For detection of free Ub by western blotting use Tris-Tricine SDS-PAGE and autoclaved nitrocellulose filters after the transfer and before blocking and addition of anti-Ub antibodies. Details on western blotting procedures are found in Mimnaugh et al., (1999 and 2002).

    Western Blot Dilution: 1:200 - 1:1000

    ELISA Dilution: 1:1,000 - 1:5,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Mimnaugh, Bonvini, Neckers: "The measurement of ubiquitin and ubiquitinated proteins." in: Electrophoresis, Vol. 20, Issue 2, pp. 418-28, (1999) (PubMed).

  • 抗原
    Ubiquitin B (UBB)
    别名
    UBB (UBB 产品)
    背景
    Background: Ubiquitin (Ub) is a small, 76-residue, protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. Free Ub is a very compact and stable molecule that is easily refolded after being denatured. It is therefore recommended that for detection of free Ub on Westerns, the Tris-Tricine SDS-PAGE is used and nitrocellulose filters are autoclaved after the transfer and before blocking and addition of anti-Ub antibodies. The C-terminus of ubiquitin forms an isopeptide bond with the e-amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein's function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction.  Conjugation of ubiquitin (Ub) involves a three-step mechanism whereby specific enzymes (or enzyme complexes) activate and covalently link Ub to their substrates.
    基因ID
    7314
    NCBI登录号
    NP_001268645
    UniProt
    P0CG47
    途径
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway
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