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AKT1 抗体 (pSer473) (Atto 594)

Rockland 200-355-268 AKT1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, FACS, FM, FLISA 宿主: 小鼠 Monoclonal 17F6-B11 Atto 594
Rockland
产品编号 ABIN1043756
Supplier Product No.: 200-355-268
发货至: 中国
  • 抗原 See all AKT1 抗体
    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
    抗原表位
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    pSer473
    适用
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    人, 小鼠, 大鼠, 猴
    宿主
    • 404
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    小鼠
    克隆类型
    • 368
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    单克隆
    标记
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    This AKT1 antibody is conjugated to Atto 594
    应用范围
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    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA
    Supplier Product No.
    200-355-268
    Supplier
    Rockland
    原理
    AKT phospho S473 ATTO594 Conjugated Antibody
    交叉反应 (详细)
    This antibody is specific for human and mouse AKT protein phosphorylated at S473.
    产品特性
    Synonyms: mouse anti-AKT pS473 ATTO 594 conjugated Antibody, ATTO 594 conjugated mouse anti-AKT pS473 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, AT594, ATTO 594, ATTO-TEC 594
    纯化方法
    Anti-AKT pS473 Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.
    免疫原

    Immunogen: Akt phospho S473 ATTO594 Conjugated Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.

    Immunogen Type: Conjugated Peptide

    克隆位点
    17F6-B11
    亚型
    IgG1 kappa
    Labeling Ratio
    2.5
    Top Product
    Discover our top product AKT1 Primary Antibody
  • 应用备注

    Flow Cytometry Dilution: User Optimized

    Application Note: Anti-AKT pS473 Antibody ATTO 594 Conjugated is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This antibody has been tested in fluorescent western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: >1:10,000

    FLISA Dilution: >1:20,000

    ELISA Dilution: 1:20,000

    IF Microscopy Dilution: >1:5,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, (2001) (PubMed).

    Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, (2001) (PubMed).

    Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, (1991) (PubMed).

    Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, (1987) (PubMed).

  • 抗原
    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
    别名
    AKT1 (AKT1 产品)
    背景
    Background: Anti-AKT antibody detects AKT which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Phospho AKT antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
    基因ID
    207, 62241011
    UniProt
    P31749
    途径
    PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
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