我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

PDCD4 抗体 (pSer457) (Biotin)

Name: PDCD4 抗体 (pSer457) (Biotin)
SKU: ABIN1043755
Availability: OutOfStock

Rockland 200-306-964 PDCD4 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, ELISA, IP 宿主: 小鼠 Monoclonal 9G6 Biotin

Western Blot of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. (PDCD4 抗体 (pSer457) (Biotin))

Image no. 2 for anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibody (Biotin) (ABIN1043755) (PDCD4 抗体 (pSer457) (Biotin))

2 images

(2 references)

产品编号 ABIN1043755

Supplier Product No.: 200-306-964

发货至: 中国

Datasheet as PDF

抗原 See all PDCD4 抗体

PDCD4 (Programmed Cell Death 4 (PDCD4))
抗原表位
15

8

7

7

7

5

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
pSer457
适用
96

62

50

6

5

4

4

3

3

3

3

3

2

1

1
人, 小鼠, 大鼠, 非洲爪蟾
宿主
90

4

2

1
小鼠
Compatible Secondaries
克隆类型
86

11
单克隆
标记
59

3

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This PDCD4 antibody is conjugated to Biotin
应用范围
73

41

30

14

14

14

12

8

6

3

3

2

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.

200-306-964

Supplier

Rockland

原理

Pdcd4 phospho S457 Antibody Biotin Conjugated

交叉反应（详细）

This antibody is specific for human Pdcd4 protein phosphorylated at Ser457.

产品特性

Synonyms: mouse anti-Pdcd4 pS457 Antibody biotin conjugation, biotin conjugated mouse anti-Pdcd4 pS457 Antibody, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

纯化方法

Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography.

免疫原

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
Immunogen Type: Conjugated Peptide

克隆位点

9G6

亚型

IgG1 kappa

Top Product

Discover our top product PDCD4 Primary Antibody
anti-Programmed Cell Death 4 (PDCD4) (C-Term) antibody
PDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibody
PDCD4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 1-100) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibody
PDCD4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (N-Term) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (pSer67) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) antibody
PDCD4 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 121-149) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB33836 unconjugated

anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibody
PDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9G6 unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 1-260) antibody
PDCD4 适用: 人 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Application Note: Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated has been tested by ELISA and western blotting and is suitable for immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Western Blot Dilution: 1:2,000 - 1:10,000
Immunoprecipitation Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Huang, Wu, Loeb, Hsu, Heidersbach, Brincat, Horiuchi, Lebbink, Mo, Goga, McManus: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).

Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, (2005) (PubMed).
抗原

PDCD4 (Programmed Cell Death 4 (PDCD4))

别名

PDCD4 (PDCD4 产品)

背景

Background: Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

基因ID

27250, 21735596

UniProt

Q53EL6