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beta Actin 抗体 (N-Term)

Name: beta Actin 抗体 (N-Term)
SKU: ABIN1043747
Availability: OutOfStock

Rockland 200-301-e69 ACTB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 3H2-E4-E12 unconjugated

Western Blot of Mouse anti-N-Term Beta Actin Monoclonal Antibody. (beta Actin 抗体 (N-Term))

Western Blot of Mouse anti-beta Actin antibody. (beta Actin 抗体 (N-Term))

Immunohistochemistry of Mouse anti-Beta Actin N-Term antibody. (beta Actin 抗体 (N-Term))

IHC

3 images

(1 reference)

产品编号 ABIN1043747

Supplier Product No.: 200-301-e69

发货至: 中国

Datasheet as PDF

抗原 See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))
抗原表位
40

16

15

6

6

5

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
181

130

110

55

42

42

37

27

25

23

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
116

81

5

1

1
小鼠
Compatible Secondaries
克隆类型
113

89
单克隆
标记
129

13

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
应用范围
171

73

53

47

33

31

26

22

13

13

8

6

6

6

5

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.

200-301-e69

Supplier

Rockland

原理

Beta Actin Antibody

交叉反应（详细）

beta Actin antibody is specific for human and mouse ß Actin protein.

产品特性

Synonyms: mouse anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

纯化方法

Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.

过滤

Sterile filtered

免疫原

Immunogen: Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.
Immunogen Type: Conjugated Peptide

克隆位点

3H2-E4-E12

亚型

IgG2a lambda

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应用备注

Immunohistochemistry Dilution: User Optimized
Application Note: Anti-beta Actin antibody has been tested for use in ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1:5000-1:50000

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store Anti-Beta Actin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Carvajal, Vallejos, Walters, Contreras, Hertz, Olivares, Cáceres, Pino, Letelier, Thompson, López-Lastra: "Structural domains within the HIV-1 mRNA and the ribosomal protein S25 influence cap-independent translation initiation." in: The FEBS journal, Vol. 283, Issue 13, pp. 2508-27, (2017) (PubMed).
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

ACTB (ACTB 产品)

背景

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

基因ID

11461

NCBI登录号

NP_001092

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction