LEFTY2 抗体
(3 references)
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- 抗原 See all LEFTY2 抗体
- LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LEFTY2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-301-279
- Supplier
- Rockland
- 原理
- LEFTY A Antibody
- 交叉反应 （详细）
- No reactivity occurs against 6X HIS tag.
- 产品特性
- Synonyms: mouse anti-leftyA antibody, mouse anti-protein lefty 2 antibody, Left-right determination factor 2 antibody, Protein lefty-2 antibody, Left-right determination factor A antibody, Protein lefty-A antibody, Transforming growth factor beta-4 antibody, TGF-beta-4 antibody, Endometrial bleeding-associated factor antibody
- 纯化方法
- This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
Immunogen Type: Recombinant Protein
- 克隆位点
- 7C5G1H6H10
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product LEFTY2 Primary Antibody
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anti-Left-Right Determination Factor 2 (LEFTY2) (AA 57-366) antibody
LEFTY2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Left-Right Determination Factor 2 (LEFTY2) (C-Term) antibodyLEFTY2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Left-Right Determination Factor 2 (LEFTY2) antibodyLEFTY2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB52667 unconjugated
anti-Left-Right Determination Factor 2 (LEFTY2) (AA 1-366) antibodyLEFTY2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Left-Right Determination Factor 2 (LEFTY2) (N-Term) antibodyLEFTY2 适用: 人, 大鼠, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Left-Right Determination Factor 2 (LEFTY2) (Internal Region) antibodyLEFTY2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Left-Right Determination Factor 2 (LEFTY2) antibodyLEFTY2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Left-Right Determination Factor 2 (LEFTY2) (N-Term) antibodyLEFTY2 适用: 人, 小鼠, 大鼠, Pig, Cow, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Left-Right Determination Factor 2 (LEFTY2) (AA 1-366) antibodyLEFTY2 适用: 人 WB 宿主: 兔 Polyclonal RB52397 unconjugated
anti-Left-Right Determination Factor 2 (LEFTY2) (AA 77-180) antibodyLEFTY2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1H6 unconjugated
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- 应用备注
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Application Note: This antibody has been tested by ELISA and western blotting. The antibody may be used for other applications, such as RIA, immunohistochemistry or immunoprecipitation, but specific reaction conditions have not been developed.
Western Blot Dilution: 1:2,000
ELISA Dilution: 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21397-404, (2001) (PubMed).
: "Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11376-81, (1999) (PubMed).
: "lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal." in: Cell, Vol. 94, Issue 3, pp. 287-97, (1998) (PubMed).
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: "Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21397-404, (2001) (PubMed).
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- 抗原
- LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))
- 别名
- LEFTY2 (LEFTY2 产品)
- 背景
- Background: During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.
- 基因ID
- 7044
- NCBI登录号
- NP_001165896
- UniProt
- O00292
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