LIM Domain Binding 1 Protein 抗体 (AA 361-373)
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- 抗原 See all LIM Domain Binding 1 Protein (LDB1) 抗体
- LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))
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抗原表位
- AA 361-373
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LIM Domain Binding 1 Protein antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 过滤
- Sterile filtered
- 免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 361-373 of mouse LDB1 protein.
- 亚型
- IgG
- Top Product
- Discover our top product LDB1 Primary Antibody
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- 应用备注
- This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using 20µg for 10E8 cells for ChIP.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.80 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))
- 别名
- LDB1 (LDB1 产品)
- 别名
- CLIM2 antibody, NLI antibody, chi antibody, clim2 antibody, nli antibody, xldb1 antibody, ldb1 antibody, LDB1 antibody, ldb4 antibody, ldb4b antibody, LIM domain binding 1 antibody, LIM domain binding 1 L homeolog antibody, LIM-domain binding 1b antibody, LIM domain binding protein 1 antibody, LIM domain binding 1a antibody, Ldb1 antibody, LDB1 antibody, ldb1.L antibody, ldb1b antibody, xldb1 antibody, ldb1a antibody
- 背景
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LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.
Synonyms: LDB1, CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. - 基因ID
- 16825, 6754520
- UniProt
- P70662
- 途径
- Stem Cell Maintenance, Chromatin Binding
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