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- 抗原 See all KLH 抗体
- KLH (Keyhole Limpet Hemocyanin (KLH))
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适用
- Keyhole Limpet
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This KLH antibody is conjugated to HRP
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应用范围
- ELISA, Western Blotting (WB)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
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KLH (Keyhole Limpet Hemocyanin
Immunogentype:Native - 亚型
- IgG
- Top Product
- Discover our top product KLH Primary Antibody
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anti-Keyhole Limpet Hemocyanin (KLH) antibody
KLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Keyhole Limpet Hemocyanin (KLH) antibody (FITC)KLH 适用: Keyhole Limpet ELISA, WB, FM 宿主: 兔 Polyclonal FITC
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Rhodamine)KLH 适用: Keyhole Limpet ELISA, WB, FM 宿主: 兔 Polyclonal Rhodamine
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Alkaline Phosphatase (AP))KLH 适用: Keyhole Limpet ELISA 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Biotin)KLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal Biotin
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Texas Red (TR))KLH 适用: Keyhole Limpet ELISA, WB, FM 宿主: 兔 Polyclonal Texas Red (TR)
anti-Keyhole Limpet Hemocyanin (KLH) antibodyKLH 适用: Keyhole Limpet ELISA, WB, IL 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Anti-KLH Antibody Peroxidase Conjugated antibody has been assayed against 1.0 ug of KLH in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 is suggested for this product. Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Peroxidase conjugated anti-KLH antibody is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Gentamicin sulfate
- 注意事项
- Do NOT add Sodium Azide!
- 储存条件
- 4 °C
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- 抗原
- KLH (Keyhole Limpet Hemocyanin (KLH))
- Abstract
- KLH 产品
- 背景
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KLH Antibody, Peroxidase Conjugated, detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Synonyms: HRP, Keyhole Limpet Hemocyanin
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