(1 reference)-
- 抗原 See all KLH 抗体
- KLH (Keyhole Limpet Hemocyanin (KLH))
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适用
- Keyhole Limpet
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This KLH antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
- 过滤
- Sterile filtered
- 免疫原
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KLH (Keyhole Limpet Hemocyanin)
Immunogentype:Native - 亚型
- IgG
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anti-Keyhole Limpet Hemocyanin (KLH) antibody (FITC)
KLH 适用: Keyhole Limpet ELISA, WB, FM 宿主: 兔 Polyclonal FITC
anti-Keyhole Limpet Hemocyanin (KLH) antibody (HRP)KLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal HRP
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Rhodamine)KLH 适用: Keyhole Limpet ELISA, WB, FM 宿主: 兔 Polyclonal Rhodamine
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Alkaline Phosphatase (AP))KLH 适用: Keyhole Limpet ELISA 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Biotin)KLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal Biotin
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Texas Red (TR))KLH 适用: Keyhole Limpet ELISA, WB, FM 宿主: 兔 Polyclonal Texas Red (TR)
anti-Keyhole Limpet Hemocyanin (KLH) antibodyKLH 适用: Keyhole Limpet ELISA, WB, IL 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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: "Human T-lymphotropic virus type-1 p30 alters cell cycle G2 regulation of T lymphocytes to enhance cell survival." in: Retrovirology, Vol. 4, pp. 49, (2007) (PubMed).
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: "Human T-lymphotropic virus type-1 p30 alters cell cycle G2 regulation of T lymphocytes to enhance cell survival." in: Retrovirology, Vol. 4, pp. 49, (2007) (PubMed).
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- 抗原
- KLH (Keyhole Limpet Hemocyanin (KLH))
- Abstract
- KLH 产品
- 背景
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KLH Antibody detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Synonyms: Keyhole Limpet Hemocyanin, KLH, Anti-KLH antibody
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