ISG15 抗体
(6 references)
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- 抗原 See all ISG15 抗体
- ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ISG15 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 200-401-438
- Supplier
- Rockland
- 原理
- ISG15 Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
- 产品特性
- Synonyms: rabbit anti-ISG15 Antibody, G1P2 antibody, IFI 15 antibody, IFI15 antibody, Interferon alpha inducible protein antibody, Interferon induced 15 kDa protein antibody
- 纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human ISG15 protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product ISG15 Primary Antibody
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anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157) antibody
ISG15 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-165) antibodyISG15 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-165) antibodyISG15 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-ISG15 Ubiquitin-Like Modifier (ISG15) (Middle Region) antibodyISG15 适用: 人, Cow, 犬, 马, Pig, 兔, 绵羊, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 31-61) antibodyISG15 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ISG15 Ubiquitin-Like Modifier (ISG15) antibodyISG15 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 31-61), (N-Term) antibodyISG15 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB02791 unconjugated
anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 39-68), (N-Term) antibodyISG15 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-ISG15 Ubiquitin-Like Modifier (ISG15) antibodyISG15 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-ISG15 Ubiquitin-Like Modifier (ISG15) (C-Term) antibodyISG15 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This purified polyclonal antibody reacts with human ISG15 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates), especially at lower dilutions. An 18.5 kDa band corresponding to human ISG15 is detected. IFNα or IFNß stimulated HeLa cell lysates can be used as a positive control.
Western Blot Dilution: 1:200 - 1:1,000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 5.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Emerging Alphaviruses Are Sensitive to Cellular States Induced by a Novel Small-Molecule Agonist of the STING Pathway." in: Journal of virology, Vol. 92, Issue 6, (2018) (PubMed).
: "Comparative proteomic analyses demonstrate enhanced interferon and STAT-1 activation in reovirus T3D-infected HeLa cells." in: Frontiers in cellular and infection microbiology, Vol. 5, pp. 30, (2015) (PubMed).
: "Alterations in the levels of vesicular trafficking proteins involved in HIV replication in the brains and CSF of patients with HIV-associated neurocognitive disorders." in: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, Vol. 8, Issue 5, pp. 1197-209, (2014) (PubMed).
: "HeLa cell response proteome alterations induced by mammalian reovirus T3D infection." in: Virology journal, Vol. 10, pp. 202, (2013) (PubMed).
: "Quantification of the host response proteome after mammalian reovirus T1L infection." in: PLoS ONE, Vol. 7, Issue 12, pp. e51939, (2013) (PubMed).
: "Deubiquitylase, deSUMOylase, and deISGylase activity microarrays for assay of substrate preference and functional modifiers." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 1, pp. M110.002402, (2011) (PubMed).
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: "Emerging Alphaviruses Are Sensitive to Cellular States Induced by a Novel Small-Molecule Agonist of the STING Pathway." in: Journal of virology, Vol. 92, Issue 6, (2018) (PubMed).
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- 抗原
- ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))
- 别名
- ISG15 (ISG15 产品)
- 背景
- Background: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. ISG15 (Interferon Stimulating Gene-15) shows no amino acid sequence homology to cytokines and is synthesized as a precursor that is activated through processing by a thiol protease. ISG15 is secreted by monocytes and lymphocytes. Synthesis is induced in response to IFN-a or IFN-b or IFN-, but not IFN-g. ISG15 expression is induced also by overexpression of some interferon regulatory factors that have been shown to play a role in the transcriptional regulation of IFN genes. ISG15 is secreted also by cell lines of monocyte (U937 cell line), T-lymphocyte, B-lymphocyte (DAUDI cells), human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated, among other things, with alterations in the expression of ISG15. Intracellularly ISG15 has been shown to function as a ubiquitin homologue. It is known also as UCRP (ubiquitin cross-reactive protein). Serpin 2a (spi2a), a member of the serine protease inhibitor (serpin) protein family that is highly induced in macrophages during bacillus Calmette-Guerin infection has been shown to bind ISG15. ISG15 has been shown to modulate immune cell function. It possesses activities of cytokines and induces production of IFN-g. It enhances proliferation and functions of natural killer and LAK cells.
- 基因ID
- 9636, 4826774
- UniProt
- P05161
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