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CD63 抗体 (Biotin)

Name: CD63 抗体 (Biotin)
Brand: antibodies-online
SKU: ABIN1027697
Availability: OutOfStock

CD63 适用: 人 FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal MEM-259 Biotin

Flow cytometry surface staining pattern of IgE stimulated human peripheral whole blood stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC). (CD63 抗体 (Biotin))

FACS

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue). (CD63 抗体 (Biotin))

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63 (CD63 抗体 (Biotin))

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 FITC. (CD63 抗体 (Biotin))

FACS

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC) and anti-human CD203c (NP4D6) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD63 抗体 (Biotin))

FACS

Separation of CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of IgE stimulated human peripheral whole blood using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC). (CD63 抗体 (Biotin))

FACS

7 images

(3 references)

产品编号 ABIN1027697

发货至: 中国

Datasheet as PDF

抗原 See all CD63 抗体

CD63
适用
122

47

8

3

2

1
人
宿主
71

60

4

2

1
小鼠
Compatible Secondaries
克隆类型
89

47

2
单克隆
标记
66

11

10

8

7

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This CD63 antibody is conjugated to Biotin
应用范围
86

60

49

41

35

28

26

16

16

11

5

3

2

2

2

2

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

特异性

The antibody MEM-259 reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

免疫原

HPB-ALL T cell line

克隆位点

MEM-259

亚型

IgG1

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anti-CD63 (CD63) (AA 120-175) antibody
CD63 适用: 人, 小鼠, 大鼠, 猴, 犬 FACS, WB, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-CD63 (CD63) antibody
CD63 适用: 人 FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal MEM-259 unconjugated

anti-CD63 (CD63) antibody (APC)
CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 APC

anti-CD63 (CD63) (AA 103-203) antibody
CD63 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 10F11E6 unconjugated

anti-CD63 (CD63) (AA 103-203) antibody
CD63 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 1H1E11 unconjugated

anti-CD63 (CD63) antibody
CD63 适用: 人, 小鼠 FACS, WB, IHC, IF, ISt, StM 宿主: 小鼠 Monoclonal MX-49-129-5 unconjugated

anti-CD63 (CD63) antibody (PE)
CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 PE

anti-CD63 (CD63) antibody (PerCP)
CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 PerCP

anti-CD63 (CD63) (AA 103-203) antibody
CD63 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1E8D4 unconjugated

anti-CD63 (CD63) (AA 163-190), (C-Term) antibody
CD63 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB26813 unconjugated

应用备注

Flow cytometry: Recommended dilution: 1-3 μg/mL, positive material: activated platelets, neutrophils and basophils.

说明

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Luddi, Zarovni, Maltinti, Governini, Leo, Cappelli, Quintero, Paccagnini, Loria, Piomboni: "Clues to Non-Invasive Implantation Window Monitoring: Isolation and Characterisation of Endometrial Exosomes." in: Cells, Vol. 8, Issue 8, (2020) (PubMed).

Heneberg, Riegerová, Kučera: "Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142953, (2015) (PubMed).

Cerny, Feng, Yu, Miyake, Borgonovo, Klumperman, Meldolesi, McNeil, Kirchhausen: "The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing." in: EMBO reports, Vol. 5, Issue 9, pp. 883-8, (2004) (PubMed).
抗原

CD63

别名

CD63 (CD63 产品)

别名

C75951 antibody, ME491 antibody, Tspan30 antibody, LAMP-3 antibody, MLA1 antibody, OMA81H antibody, TSPAN30 antibody, TSP29FA antibody, CD63 antigen antibody, CD63 molecule antibody, Cd63 molecule antibody, Cd63 antibody, CD63 antibody

背景

CD63 Molecule,CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.,OMA81H, Granulophysin, Tetraspanin-30, Tspan-30, MLA1, ME491, LAMP-3, OMA81H, TSPAN30

基因ID

967

UniProt

P08962