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kappa Light Chain 抗体 (PE)

Name: kappa Light Chain 抗体 (PE)
Brand: antibodies-online
SKU: ABIN1027676
Availability: OutOfStock

适用: 人 FACS 宿主: 小鼠 Monoclonal TB28-2 PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (TB28-2) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (kappa Light Chain 抗体 (PE))

FACS

Flow cytometry multicolor surface staining of human CD19 positive B cells using anti-human Ig Kappa Light Chain (TB28-2) PE (c = 5 μg/mL) and anti-human Ig Lambda Light Chain (1-155-2) FITC (c = 5 μg/mL) antibodies. (kappa Light Chain 抗体 (PE))

FACS

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human Ig Kappa Light Chain (TB28-2) PE antibody (10 μL reagent / 100 μL of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μL reagent / 100 μL of peripheral whole blood). (kappa Light Chain 抗体 (PE))

FACS

Separation of human Ig Lambda Kappa Chain positive B cells (red-filled) from Ig Kappa Light Chain negative B cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (TB28-2) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (kappa Light Chain 抗体 (PE))

FACS

4 images

(5 references)

产品编号 ABIN1027676

发货至: 中国

Datasheet as PDF

抗原 See all kappa Light Chain 抗体

kappa Light Chain
适用
33

19

1
人
宿主
24

23

2

1

1
小鼠
Compatible Secondaries
克隆类型
26

25
单克隆
标记
14

5

4

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

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1

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1

1

1
This kappa Light Chain antibody is conjugated to PE
应用范围
31

22

13

13

10

10

9

6

4

4

3

2

2
Flow Cytometry (FACS)

特异性

The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Human IgG-kappa myeloma protein

克隆位点

TB28-2

亚型

IgG1 kappa

Top Product

Discover our top product kappa Light Chain Primary Antibody
anti-kappa Light Chain antibody (FITC)
适用: 人 FACS 宿主: 小鼠 Monoclonal A8B5 FITC

anti-kappa Light Chain antibody
适用: 人 FACS, IHC (p), ELISA, IHC (fro) 宿主: 小鼠 Monoclonal A8B5 unconjugated

anti-kappa Light Chain antibody
适用: 人 FACS, WB 宿主: 小鼠 Monoclonal TB28-2 unconjugated

anti-kappa Light Chain antibody
适用: 人 FACS, WB, IHC (p), ELISA, ICC, IP 宿主: 小鼠 Monoclonal MEM-09 unconjugated

anti-kappa Light Chain antibody (Biotin)
适用: 人 FACS 宿主: 小鼠 Monoclonal TB28-2 Biotin

anti-kappa Light Chain antibody (APC)
适用: 人 FACS 宿主: 小鼠 Monoclonal TB28-2 APC

anti-kappa Light Chain (AA 1-60) antibody
适用: 小鼠 WB, IHC (p), ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-kappa Light Chain antibody (mFluor™450)
GMP Grade 适用: 人 FACS 宿主: 小鼠 Monoclonal TB28-2 mFluor™450

anti-kappa Light Chain antibody (PE-Cy7)
GMP Grade 适用: 人 FACS 宿主: 小鼠 Monoclonal TB 28-2 PE-Cy7

anti-kappa Light Chain (AA 1-60) antibody (Biotin)
适用: 小鼠 WB, IHC (p), ELISA, IHC (fro) 宿主: 兔 Polyclonal Biotin

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Jourdan, Caraux, De Vos, Fiol, Larroque, Cognot, Bret, Duperray, Hose, Klein: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." in: Blood, Vol. 114, Issue 25, pp. 5173-81, (2009) (PubMed).

Böttcher, Ritgen, Buske, Gesk, Klapper, Hoster, Hiddemann, Unterhalt, Dreyling, Siebert, Kneba, Pott et al.: "Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for ..." in: Haematologica, Vol. 93, Issue 4, pp. 551-9, (2008) (PubMed).

Karandikar, Aquino, McKenna, Kroft: "Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis." in: American journal of clinical pathology, Vol. 116, Issue 2, pp. 204-10, (2001) (PubMed).

Nakamura, Kubagawa, Cooper: "Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8522-6, (1992) (PubMed).

Kiyotaki, Cooper, Bertoli, Kearney, Kubagawa: "Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4150-8, (1987) (PubMed).
抗原

kappa Light Chain

别名

Kappa light chains (kappa Light Chain 产品)

别名

LOC100037688 antibody, B128L antibody, B55L antibody, IgK antibody, TCRAV31S1 antibody, TCRAV40S1 antibody, kappa light chain antibody, immunoglobulin kappa chain variable 8 (V8) antibody, T cell receptor alpha variable 40 antibody, LOC100037688 antibody, Igk-V8 antibody, TRAV40 antibody

背景

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin kappa, Igk