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CD25 抗体 (PerCP)

Name: CD25 抗体 (PerCP)
Brand: antibodies-online
SKU: ABIN1027672
Availability: OutOfStock

IL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 PerCP

Separation of CD25 positive cells stained using anti-human CD25 (MEM-181) PerCP antibody (10 μL reagent per million cells in 100 μL of cell suspension, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) PerCP antibody (concentration in sample 3 μg/mL, same as CD25 PerCP concentration, black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells. (CD25 抗体 (PerCP))

FACS

Surface staining of human PBMC with anti-human CD25 (MEM-181) FITC. (CD25 抗体 (PerCP))

FACS

Surface staining of PHA-stimulated (3 days) human PBMC with anti-CD25 (MEM-181) PerCP. (CD25 抗体 (PerCP))

FACS

3 images

(1 reference)

产品编号 ABIN1027672

发货至: 中国

Datasheet as PDF

抗原 See all CD25 (IL2RA) 抗体

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
适用
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110

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人
宿主
129

85

77

2

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1
小鼠
Compatible Secondaries
克隆类型
212

79

2
单克隆
标记
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32

26

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This CD25 antibody is conjugated to PerCP
应用范围
206

85

52

49

39

36

19

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14

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Flow Cytometry (FACS)

特异性

The antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells), it is lost on resting B and T lymphocytes.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

PHA-activated peripheral blood leucocytes

克隆位点

MEM-181

亚型

IgG1

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应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).
抗原

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

别名

CD25 (IL2RA 产品)

别名

CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody

背景

Interleukin 2 receptor subunit alpha,CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 Molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.,IL-2R α, chain, IL-2-RA, IL2-RA, p55, TAC antigen, IDDM10, TCGFR

基因ID

3559

UniProt

P01589

途径

JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation