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CD99 抗体 (PE)

Name: CD99 抗体 (PE)
Brand: antibodies-online
SKU: ABIN1027670
Availability: OutOfStock

CD99 适用: 人 FACS 宿主: 小鼠 Monoclonal 3B2-TA8 PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD99 (3B2/TA8) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (CD99 抗体 (PE))

FACS

Surface staining of human peripheral blood cells with anti-human CD99 (3B2/TA8) PE. (CD99 抗体 (PE))

FACS

Separation of human CD99 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD99 (3B2/TA8) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (CD99 抗体 (PE))

FACS

3 images

(2 references)

产品编号 ABIN1027670

发货至: 中国

Datasheet as PDF

抗原 See all CD99 抗体

CD99
适用
100

24

9

6

1
人
宿主
72

47

5
小鼠
Compatible Secondaries
克隆类型
68

56
单克隆
标记
48

12

10

8

4

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD99 antibody is conjugated to PE
应用范围
61

52

35

29

22

17

14

13

12

12

5

4

4

1

1
Flow Cytometry (FACS)

特异性

The mouse monoclonal antibody 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on the surface of many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Human thymocytes

克隆位点

3B2-TA8

亚型

IgG2a kappa

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CD99 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5C8 unconjugated

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CD99 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal MIC2-877 unconjugated

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Brémond, Meynet, Mahiddine, Coito, Tichet, Scotlandi, Breittmayer, Gounon, Gleeson, Bernard, Bernard: "Regulation of HLA class I surface expression requires CD99 and p230/golgin-245 interaction." in: Blood, Vol. 113, Issue 2, pp. 347-57, (2009) (PubMed).

Kueng, Leb, Haiderer, Raposo, Thery, Derdak, Schmetterer, Neunkirchner, Sillaber, Seed, Pickl: "General strategy for decoration of enveloped viruses with functionally active lipid-modified cytokines." in: Journal of virology, Vol. 81, Issue 16, pp. 8666-76, (2007) (PubMed).
抗原

CD99

别名

CD99 (CD99 产品)

别名

1110061M03Rik antibody, 2410026K10Rik antibody, D4 antibody, Pilr-l antibody, pilr-1 antibody, HBA71 antibody, MIC2 antibody, MIC2X antibody, MIC2Y antibody, MSK5X antibody, CD99 antigen antibody, CD99 molecule (Xg blood group) antibody, CD99 molecule antibody, Cd99 antibody, CD99 antibody

物质类

Viral Protein

背景

CD99 Molecule (Xg blood group),CD99 is a ubiquitous transmembrane type I sialoglycoprotein of a unique and poorly characterized protein family. CD99 is heavily O-glycosylated and was described as a T cell costimulator and strong activator of integrin-mediated actin cytoskeleton assembly, promoting cell adhesion and homotypic aggregation, immediate arrest on an inflamed vascular endothelium, and cell migration through it. Ligation of CD99 under some conditions can lead to apoptosis. Originally CD99 was described as a human thymus leukemia antigen, an Ewing´s sarcoma-specific membrane marker, and an adhesion molecule involved in spontaneous rosette formation of T cells with erythrocytes.,MIC2, HBA71, blood group Xg

基因ID

4267

UniProt

P14209