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His Tag 抗体 (Biotin)

Name: His Tag 抗体 (Biotin)
Brand: antibodies-online
SKU: ABIN100493
Availability: OutOfStock

WB, ELISA, IP 宿主: 兔 Polyclonal Biotin

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. (His Tag 抗体 (Biotin))

1 image

(7 references)

产品编号 ABIN100493

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

请咨询
宿主
91

59

11

9

5

4

1

1
兔
Compatible Secondaries
克隆类型
93

75

10
多克隆
标记
79

21

13

12

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is conjugated to Biotin
应用范围
140

90

38

27

24

18

15

14

14

13

13

7

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP)

序列

HHHHHH

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

亚型

IgG

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应用备注

Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

限制

仅限研究用
状态

Lyophilized

溶解方式

Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C
Blomberg, Rizwan, Böhlin-Wiener, Elfaitouri, Julin, Zachrisson, Rosén, Gottfries: "Antibodies to Human Herpesviruses in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Patients." in: Frontiers in immunology, Vol. 10, pp. 1946, (2019) (PubMed).

Place, Williamson, Yuzefpolskiy, Katkere, Sarkar, Kalia, Kirimanjeswara: "Development of a novel Francisella tularensis Live Vaccine Strain expressing ovalbumin provides insight into antigen-specific CD8+ T cell responses." in: PLoS ONE, Vol. 12, Issue 12, pp. e0190384, (2018) (PubMed).

Rönnberg, Gustafsson, Vapalahti, Emmerich, Lundkvist, Schmidt-Chanasit, Blomberg: "Compensating for cross-reactions using avidity and computation in a suspension multiplex immunoassay for serotyping of Zika versus other flavivirus infections." in: Medical microbiology and immunology, Vol. 206, Issue 5, pp. 383-401, (2018) (PubMed).

Song, Chang, Ma, Tan: "Single-Molecule Fluorescence Methods to Study Plant Hormone Signal Transduction Pathways." in: Frontiers in plant science, Vol. 8, pp. 1888, (2017) (PubMed).

Rönnberg, Vapalahti, Goeijenbier, Reusken, Gustafsson, Blomberg, Lundkvist: "Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array." in: Infection ecology & epidemiology, Vol. 7, Issue 1, pp. 1350086, (2017) (PubMed).

Rizwan, Rönnberg, Cistjakovs, Lundkvist, Pipkorn, Blomberg: "Serology in the Digital Age: Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays." in: Microarrays (Basel, Switzerland), Vol. 5, Issue 3, (2016) (PubMed).

Yi, Ba, Zhang, Ho, Chen: "Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain." in: Journal of virology, Vol. 79, Issue 18, pp. 11638-46, (2005) (PubMed).
抗原

His Tag

别名

6 X His Tag (His Tag 产品)

物质类

Tag

背景

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.