我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

His Tag 抗体

Name: His Tag 抗体
SKU: ABIN100492
Availability: OutOfStock

Rockland 600-401-382 WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated

A knock-in mouse line with NALCN tagged with GFP, HA, and His tags (NALCN-GFP-HA-His mice). (His Tag 抗体)

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. (His Tag 抗体)

Western blot of Rabbit Anti-6xHIS Epitope Tag antibody. (His Tag 抗体)

Crude extracts prepared from Salmonella strains with the phoR-8×Myc gene expressing PhoU-His, PhoUR184A-His, PhoUR184G-His, or the empty vector (pBAD33) were detected with anti-Myc (top) and anti-His (bottom) antibodies. (His Tag 抗体)

$Eluted fractions prepared from strains listed above were detected with anti-Myc (top) and anti-His (bottom) antibodies after immunoprecipitation with anti-Myc antibody-coated beads. (His Tag 抗体)$ WB

5 images

(14 references)

产品编号 ABIN100492

Supplier Product No.: 600-401-382

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

请咨询
宿主
92

58

11

9

9

8

2

1
兔
Compatible Secondaries
克隆类型
104

73

10
多克隆
标记
88

20

13

13

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
应用范围
149

110

33

32

28

24

14

14

13

13

12

11

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Supplier Product No.

600-401-382

Supplier

Rockland

原理

6X His Epitope Tag Antibody

序列

HHHHHH

交叉反应（详细）

This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

产品特性

Synonyms: rabbit anti-6X His Epitope Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

纯化方法

Affinity purified antibody

过滤

Sterile filtered

免疫原

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Immunogen Type: Conjugated Peptide

亚型

IgG
anti-His Tag antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag (C-Term) antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 800)
WB, ELISA, DB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800

anti-His Tag antibody (Cy3)
WB, ELISA, FACS, DB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3

anti-His Tag antibody (DyLight 649)
WB, ELISA, DB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649

anti-His Tag antibody (HRP)
WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP

应用备注

Immunohistochemistry Dilution: 1:500 - 1:2,000
Application Note: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Western Blot Dilution: 1:500 - 1:1,000
ELISA Dilution: 1:10,000 - 1:40,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Mart Nez-Cruz, Romero, Hierrezuelo, Thon, de Vicente, P Rez-Garc A: "Effectors with chitinase activity (EWCAs), a family of conserved, secreted fungal chitinases that suppress chitin-triggered immunity." in: The Plant cell, Vol. 33, Issue 4, pp. 1319-1340, (2021) (PubMed).

Wie, Bharthur, Wolfgang, Narayanan, Ramsey, Aranda, Zhang, Zhou, Ren: "Intellectual disability-associated UNC80 mutations reveal inter-subunit interaction and dendritic function of the NALCN channel complex." in: Nature communications, Vol. 11, Issue 1, pp. 3351, (2020) (PubMed).

Koh, Cao, Mangos, Tardi, Dande, Lee, Samelko, Altintas, Schmitz, Lee, Mukherjee, Peev, Cimbaluk, Reiser, Hahm: "Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist." in: The Journal of clinical investigation, Vol. 129, Issue 4, pp. 1713-1726, (2019) (PubMed).

Vela-Corcía, Romero, de Vicente, Pérez-García: "Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance." in: Scientific reports, Vol. 8, Issue 1, pp. 7161, (2019) (PubMed).

Lecona, Rodriguez-Acebes, Specks, Lopez-Contreras, Ruppen, Murga, Muñoz, Mendez, Fernandez-Capetillo: "USP7 is a SUMO deubiquitinase essential for DNA replication." in: Nature structural & molecular biology, Vol. 23, Issue 4, pp. 270-7, (2016) (PubMed).

Lucchetti, Caligiuri, Toffoli, Giordano, Rizzolio: "The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor α in breast cancer." in: PLoS ONE, Vol. 8, Issue 2, pp. e55355, (2013) (PubMed).

La Montagna, Caligiuri, Maranta, Lucchetti, Esposito, Paggi, Toffoli, Rizzolio, Giordano: "Androgen receptor serine 81 mediates Pin1 interaction and activity." in: Cell cycle (Georgetown, Tex.), Vol. 11, Issue 18, pp. 3415-20, (2013) (PubMed).

Bradley, Chan, Hagood: "Effect of the GPI anchor of human Thy-1 on antibody recognition and function." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 93, Issue 3, pp. 365-74, (2013) (PubMed).

Curtis, Griffin: "The chromatin-remodeling enzymes BRG1 and CHD4 antagonistically regulate vascular Wnt signaling." in: Molecular and cellular biology, Vol. 32, Issue 7, pp. 1312-20, (2012) (PubMed).

García-López, Sánchez-Amaya, Halm, Astola, Prat et al.: "Bone morphogenetic protein 15 and growth differentiation factor 9 expression in the ovary of European sea bass (Dicentrarchus labrax): cellular localization, developmental profiles, and response to ..." in: General and comparative endocrinology, Vol. 174, Issue 3, pp. 326-34, (2012) (PubMed).

Sorrentino, Scheer, Santos, Reits, Bleijlevens, Zelcer: "Distinct functional domains contribute to degradation of the low density lipoprotein receptor (LDLR) by the E3 ubiquitin ligase inducible Degrader of the LDLR (IDOL)." in: The Journal of biological chemistry, Vol. 286, Issue 34, pp. 30190-9, (2011) (PubMed).

Niederkofler, Baeriswyl, Ott, Stoeckli: "Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance." in: Development (Cambridge, England), Vol. 137, Issue 3, pp. 427-35, (2010) (PubMed).

Monegal, Ami, Martinelli, Huang, Aliprandi, Capasso, Francavilla, Ossolengo, de Marco: "Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library." in: Protein engineering, design & selection : PEDS, Vol. 22, Issue 4, pp. 273-80, (2009) (PubMed).

Yi, Ba, Zhang, Ho, Chen: "Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain." in: Journal of virology, Vol. 79, Issue 18, pp. 11638-46, (2005) (PubMed).
抗原

His Tag

别名

6X His (His Tag 产品)

物质类

Tag

背景

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.