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His Tag 抗体

Name: His Tag 抗体
Brand: antibodies-online
SKU: ABIN100492
Availability: OutOfStock

WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated

A knock-in mouse line with NALCN tagged with GFP, HA, and His tags (NALCN-GFP-HA-His mice). (His Tag 抗体)

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. (His Tag 抗体)

Western blot of Rabbit Anti-6xHIS Epitope Tag antibody. (His Tag 抗体)

Crude extracts prepared from Salmonella strains with the phoR-8×Myc gene expressing PhoU-His, PhoUR184A-His, PhoUR184G-His, or the empty vector (pBAD33) were detected with anti-Myc (top) and anti-His (bottom) antibodies. (His Tag 抗体)

$Eluted fractions prepared from strains listed above were detected with anti-Myc (top) and anti-His (bottom) antibodies after immunoprecipitation with anti-Myc antibody-coated beads. (His Tag 抗体)$ WB

5 images

(14 references)

产品编号 ABIN100492

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

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宿主
89

59

11

9

5

4

1

1
兔
Compatible Secondaries
克隆类型
91

75

10
多克隆
标记
76

21

13

13

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
应用范围
138

88

36

24

24

16

14

14

13

13

13

7

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

序列

HHHHHH

特异性

This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

产品特性

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

过滤

Sterile filtered

免疫原

This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Immunogen Type: Peptide

亚型

IgG
anti-His Tag antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, IHC, FACS 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP

anti-His Tag (C-Term) antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 Biotin

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 649)
WB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2 DyLight 649

anti-His Tag antibody
WB, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated

应用备注

Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

有效期

12 months
Mart Nez-Cruz, Romero, Hierrezuelo, Thon, de Vicente, P Rez-Garc A: "Effectors with chitinase activity (EWCAs), a family of conserved, secreted fungal chitinases that suppress chitin-triggered immunity." in: The Plant cell, Vol. 33, Issue 4, pp. 1319-1340, (2021) (PubMed).

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La Montagna, Caligiuri, Maranta, Lucchetti, Esposito, Paggi, Toffoli, Rizzolio, Giordano: "Androgen receptor serine 81 mediates Pin1 interaction and activity." in: Cell cycle (Georgetown, Tex.), Vol. 11, Issue 18, pp. 3415-20, (2013) (PubMed).

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Niederkofler, Baeriswyl, Ott, Stoeckli: "Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance." in: Development (Cambridge, England), Vol. 137, Issue 3, pp. 427-35, (2010) (PubMed).

Monegal, Ami, Martinelli, Huang, Aliprandi, Capasso, Francavilla, Ossolengo, de Marco: "Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library." in: Protein engineering, design & selection : PEDS, Vol. 22, Issue 4, pp. 273-80, (2009) (PubMed).

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抗原

His Tag

Abstract

His Tag 产品

物质类

Tag

背景

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.