Hexokinase 抗体
适用: Saccharomyces cerevisiae
WB, ELISA, IHC
宿主: 兔
Polyclonal
unconjugated
(2 references)-
- 抗原 See all Hexokinase 抗体
- Hexokinase
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适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Hexokinase antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 交叉反应 （详细）
- Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined.
- 纯度
- Anti-Hexokinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Anti-Hexokinase Antibody was produced by repeated immunizations with yeast hexokinase protein.
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product Hexokinase Primary Antibody
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anti-Hexokinase (AA 1-917) antibody
适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D7 unconjugated
anti-Hexokinase antibody (HRP)适用: Saccharomyces cerevisiae WB, ELISA 宿主: 兔 Polyclonal HRP
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- 应用备注
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Anti-Hexokinase Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:565.000
Western Blot Dilution: 1:500 - 1:2.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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: "Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination." in: Science advances, Vol. 6, Issue 6, pp. eaay2669, (2020) (PubMed).
: "A Comprehensive Membrane Interactome Mapping of Sho1p Reveals Fps1p as a Novel Key Player in the Regulation of the HOG Pathway in S. cerevisiae." in: Journal of molecular biology, Vol. 427, Issue 11, pp. 2088-103, (2015) (PubMed).
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: "Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination." in: Science advances, Vol. 6, Issue 6, pp. eaay2669, (2020) (PubMed).
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- 抗原
- Hexokinase
- Abstract
- Hexokinase 产品
- 背景
- Anti-Hexokinase antibody detects hexokinase. Hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Anti-Hexokinase Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research. Synonyms: DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody
- 基因ID
- 852639
- UniProt
- P04807
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