Hexokinase 2 抗体
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- 抗原 See all Hexokinase 2 (HK2) 抗体
- Hexokinase 2 (HK2)
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适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Hexokinase 2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Supplier Product No.
- 200-4159-0100
- Supplier
- Rockland
- 原理
- Hexokinase Antibody
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].
- 产品特性
- Synonyms: rabbit anti-Hexokinase Antibody, DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody
- 纯化方法
- Anti-Hexokinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Anti-Hexokinase Antibody was produced by repeated immunizations with yeast hexokinase protein.
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product HK2 Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Hexokinase Antibody has been tested by western blotting and for ELISA and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1,000 - 1: 5,000
ELISA Dilution: 1:3,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination." in: Science advances, Vol. 6, Issue 6, pp. eaay2669, (2020) (PubMed).
: "A Comprehensive Membrane Interactome Mapping of Sho1p Reveals Fps1p as a Novel Key Player in the Regulation of the HOG Pathway in S. cerevisiae." in: Journal of molecular biology, Vol. 427, Issue 11, pp. 2088-103, (2015) (PubMed).
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Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination." in: Science advances, Vol. 6, Issue 6, pp. eaay2669, (2020) (PubMed).
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- 抗原
- Hexokinase 2 (HK2)
- 别名
- HXK2 (HK2 产品)
- 背景
- Background: Anti-Hexokinase antibody detects hexokinase. Hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Anti-Hexokinase Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
- 基因ID
- 852639
- UniProt
- P04806
- 途径
- PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect
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