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- 抗原 See all HA-Tag products
- HA-Tag
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HA-Tag antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunomicroscopy (IM)
- 序列
- YPYDVPDYA
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
- This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
- 亚型
- IgG
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anti-HA-Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 18B11H6 unconjugated
anti-HA-Tag antibodyWB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag antibodyWB, IF, ChIP 宿主: 兔 Polyclonal RB0411 unconjugated
anti-HA-Tag antibodyWB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag antibody (HRP)WB, ELISA, IP 宿主: 小鼠 Monoclonal 5E11D8 HRP
anti-HA-Tag antibody适用: 标记 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 4G8 unconjugated
anti-HA-Tag antibodyELISA, Coat 宿主: 小鼠 Monoclonal 16-43 unconjugated
anti-HA-Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 12CA5 unconjugated
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- 应用备注
- Anti-HA is optimally suited for monitoring the expression of HA tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- terminus of targeted proteins as is expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. Biotin conjugated anti-EPITOPE tag antibodies are generally suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- HA-Tag
- Abstract
- HA-Tag 产品
- 物质类
- Tag
- 背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
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