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GFP 抗体 (FITC)

Name: GFP 抗体 (FITC)
SKU: ABIN100088
Availability: OutOfStock

Rockland 600-102-215 GFP 适用: Aequorea victoria WB, IHC, FM, DB, FLISA 宿主: 山羊 Polyclonal FITC

Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (FITC) (ABIN100088) (GFP 抗体 (FITC))

Immunofluorescence Microscopy of GFP-GOAT-Antibody. (GFP 抗体 (FITC))

Dot Blot of Fluorescein conjugated Goat anti-GFP. (GFP 抗体 (FITC))

Western Blot of Goat anti-GFP Fluorescein Conjugated Antibody. (GFP 抗体 (FITC))

4 images

(8 references)

产品编号 ABIN100088

Supplier Product No.: 600-102-215

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
165

15

11

9
Aequorea victoria
宿主
62

57

33

13

9

5

1

1
山羊
Compatible Secondaries
克隆类型
109

68
多克隆
标记
79

11

10

9

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to FITC
应用范围
142

74

47

40

26

26

25

22

15

13

13

13

12

11

4

4

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Supplier Product No.

600-102-215

Supplier

Rockland

原理

GFP Antibody Fluorescein Conjugated

交叉反应（详细）

Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

产品特性

Synonyms: goat anti-GFP Antibody fluorescein Conjugation, FITC conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

纯化方法

GFP Fluorescein Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Recombinant Protein

亚型

IgG

Labeling Ratio

3.85

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应用备注

Immunohistochemistry Dilution: User Optimized
Application Note: Anti-GFP Fluorescein Conjugated Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: >1:10,000
FLISA Dilution: >1:20,000
IF Microscopy Dilution: 1:500 - 1:2,500
Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Perna, Alberi: "TF-ChIP Method for Tissue-Specific Gene Targets." in: Frontiers in cellular neuroscience, Vol. 13, pp. 95, (2019) (PubMed).

Hock, Maniecka, Hruska-Plochan, Reber, Laferrière, Sahadevan M K, Ederle, Gittings, Pelkmans, Dupuis, Lashley, Ruepp, Dormann, Polymenidou: "Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function." in: Cell reports, Vol. 24, Issue 4, pp. 987-1000.e7, (2018) (PubMed).

Kwon, Everetts, Wang, Wang, Della Croce, Xing, Yao: "Controlling Depth of Cellular Quiescence by an Rb-E2F Network Switch." in: Cell reports, Vol. 20, Issue 13, pp. 3223-3235, (2018) (PubMed).

Fong, Mazo, Das, Goodman, Kim, ORourke, Izquierdo, Tsou: "53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis." in: eLife, Vol. 5, (2016) (PubMed).

Doucet, Woo, Ruiz, Owens: "The touch dome defines an epidermal niche specialized for mechanosensory signaling." in: Cell reports, Vol. 3, Issue 6, pp. 1759-65, (2014) (PubMed).

Wang, Tay, Soni, Perumal, Goll, Macaluso, Asara, Amack, Tsou: "CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base." in: Nature cell biology, Vol. 15, Issue 6, pp. 591-601, (2013) (PubMed).

Guo, Maeda, Ma, Delgado, Sohn, Miers, Ko, Bannerman, Xu, Wang, Zhou, Takebayashi, Pleasure: "Macroglial plasticity and the origins of reactive astroglia in experimental autoimmune encephalomyelitis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 33, pp. 11914-28, (2011) (PubMed).

Niederkofler, Baeriswyl, Ott, Stoeckli: "Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance." in: Development (Cambridge, England), Vol. 137, Issue 3, pp. 427-35, (2010) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

背景

Background: GFP Fluorescein Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt

P42212