GFP 抗体 (Biotin)
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- 抗原 See all GFP 抗体
- GFP (Green Fluorescent Protein (GFP))
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适用
- Aequorea victoria
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GFP antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 600-106-215
- Supplier
- Rockland
- 原理
- GFP (GOAT) Antibody Biotin Conjugated
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.
- 产品特性
- Synonyms: goat anti-GFP Antibody biotin Conjugation, biotin conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
- 纯化方法
- Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
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Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product GFP Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-GFP Biotin Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:50,000 - 1:80,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store Anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage." in: EMBO reports, Vol. 20, Issue 10, pp. e48014, (2019) (PubMed).
: "Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor." in: eLife, Vol. 8, (2019) (PubMed).
: "Origin, fate and dynamics of macrophages at central nervous system interfaces. ..." in: Nature immunology, Vol. 17, Issue 7, pp. 797-805, (2017) (PubMed).
: "Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function." in: Acta neuropathologica, Vol. 130, Issue 3, pp. 389-406, (2016) (PubMed).
: "A redox signalling globin is essential for reproduction in Caenorhabditis elegans." in: Nature communications, Vol. 6, pp. 8782, (2016) (PubMed).
: "An AKT3-FOXG1-reelin network underlies defective migration in human focal malformations of cortical development." in: Nature medicine, Vol. 21, Issue 12, pp. 1445-54, (2016) (PubMed).
: "A combined transgenic proteomic analysis and regulated trafficking of neuroligin-2." in: The Journal of biological chemistry, Vol. 289, Issue 42, pp. 29350-64, (2014) (PubMed).
: "Dynamics of major histocompatibility complex class I association with the human peptide-loading complex." in: The Journal of biological chemistry, Vol. 287, Issue 37, pp. 31172-84, (2012) (PubMed).
: "Single-molecule pull-down for studying protein interactions." in: Nature protocols, Vol. 7, Issue 3, pp. 445-52, (2012) (PubMed).
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SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport." in: The EMBO journal, Vol. 39, Issue 6, pp. e102301, (2020) (PubMed).
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- 抗原
- GFP (Green Fluorescent Protein (GFP))
- 别名
- GFP (GFP 产品)
- 背景
- Background: Conjugated Anti-GFP is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
- UniProt
- P42212
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