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GFP 抗体 (Biotin)

Name: GFP 抗体 (Biotin)
SKU: ABIN100087
Availability: OutOfStock

Rockland 600-106-215 GFP 适用: Aequorea victoria WB, ELISA, IHC 宿主: 山羊 Polyclonal Biotin

Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (Biotin) (ABIN100087) (GFP 抗体 (Biotin))

Immunofluorescence Microscopy of GFP GOAT Antibody. (GFP 抗体 (Biotin))

2 images

(15 references)

产品编号 ABIN100087

Supplier Product No.: 600-106-215

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
165

15

11

9
Aequorea victoria
宿主
61

56

35

13

9

5

1

1
山羊
Compatible Secondaries
克隆类型
111

66
多克隆
标记
79

10

10

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Biotin
应用范围
142

76

46

43

30

29

25

24

16

13

13

13

13

11

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.

600-106-215

Supplier

Rockland

原理

GFP (GOAT) Antibody Biotin Conjugated

交叉反应（详细）

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.

产品特性

Synonyms: goat anti-GFP Antibody biotin Conjugation, biotin conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

纯化方法

Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Recombinant Protein

亚型

IgG

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应用备注

Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-GFP Biotin Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:50,000 - 1:80,000
Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store Anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Jongsma, Bakker, Cabukusta, Liv, van Elsland, Fermie, Akkermans, Kuijl, van der Zanden, Janssen, Hoogzaad, van der Kant, Wijdeven, Klumperman, Berlin, Neefjes: "SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport." in: The EMBO journal, Vol. 39, Issue 6, pp. e102301, (2020) (PubMed).

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抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

背景

Background: Conjugated Anti-GFP is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

UniProt

P42212