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GFP 抗体

Name: GFP 抗体
Brand: antibodies-online
SKU: ABIN100085
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated

Western Blot of Goat anti-GFP antibody. (GFP 抗体)

MSCs are traced in special organized regions. (GFP 抗体)

IHC

Immunofluorescence Microscopy of GFP-GOAT-Antibody. (GFP 抗体)

Western Blot of Rabbit anti-GFP antibody. (GFP 抗体)

Immunofluorescence Microscopy of Anti-GFP (GOAT) Antibody. (GFP 抗体)

Western Blot of Goat anti-GFP antibody with lysates. (GFP 抗体)

Western Blot of Goat anti-GFP Antibody with Serums. (GFP 抗体)

Tyrosine phosphorylation of HIPK mutants. (GFP 抗体)

Maximal activity of HIPKs depends on the activation loop tyrosine. (GFP 抗体)

Subcellular distribution of HIPK mutants. (GFP 抗体)

15 images

(178 references)

产品编号 ABIN100085

发货至: 中国

Datasheet as PDF

Key Features
- Polyclonal, unconjugated GFP antibody for reliable detection of GFP and its variants.
- Validated for Fluorescence Microscopy, ELISA, Western Blotting
- High Quality GFP antibody, cited in more than 177 PubMed References.
- Available in 10 µl and 100 µl quantities.
抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
176

15

11

9
Aequorea victoria
宿主
65

62

34

14

9

5

2

1
山羊
Compatible Secondaries
克隆类型
114

73
多克隆
标记
83

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
149

74

46

33

27

26

25

20

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

原理

Goat Anti-GFP is ideal for western blotting, ELISA, Immunohistochemistry and IP.

特异性

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.

交叉反应（详细）

wt, rGFP, eGFP

产品特性

Anti-GFP is designed to detect GFP and its variants.

纯化方法

GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

过滤

Sterile filtered

免疫原

The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogentype: Recombinant

亚型

IgG
anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC

anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)
GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

anti-Green Fluorescent Protein (GFP) (AA 1-100) antibody
GFP 适用: Aequorea victoria WB, IF 宿主: 小鼠 Monoclonal unconjugated

应用备注

ELISA : 1:10,000 - 1:30,000
IF Microscopy : 1:500
Western Blot : 1:1,000 - 1:10,000
Immunohistochemistry: 1:200 - 1:1,000
ImmunoPrecipitation: Yes

说明

This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (ABIN129564) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP (ABIN964537).
Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone.
For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.

限制

仅限研究用
状态

Liquid

浓度

1.08 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid cycles of freezing and thawing.

储存条件

-20 °C

储存方法

Store GFP antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Lapraz, Boutres, Fixary-Schuster, De Queiroz, Plaçais, Cerezo, Besse, Préat, Noselli: "Asymmetric activity of NetrinB controls laterality of the Drosophila brain." in: Nature communications, Vol. 14, Issue 1, pp. 1052, (2023) (PubMed).

Cone, Hurwitz, Lee, Yuan, Zhou, Li, Meckes: "Alix and Syntenin-1 direct amyloid precursor protein trafficking into extracellular vesicles." in: BMC molecular and cell biology, Vol. 21, Issue 1, pp. 58, (2020) (PubMed).

Kim, Kim, Choi, Lee, Lee, Im, Shin, Kim, Hong, Kim, Kim, Sung: "Downregulated miR-18b-5p triggers apoptosis by inhibition of calcium signaling and neuronal cell differentiation in transgenic SOD1 (G93A) mice and SOD1 (G17S and G86S) ALS patients." in: Translational neurodegeneration, Vol. 9, Issue 1, pp. 23, (2020) (PubMed).

Saegusa, Hosoya, Nishiyama, Saeki, Fujimoto, Okano, Fujioka, Ogawa: "Low-dose rapamycin-induced autophagy in cochlear outer sulcus cells." in: Laryngoscope investigative otolaryngology, Vol. 5, Issue 3, pp. 520-528, (2020) (PubMed).

Nkosi, Sun, Duke, Patel, Surapaneni, Singh, Meckes: "Epstein-Barr Virus LMP1 Promotes Syntenin-1- and Hrs-Induced Extracellular Vesicle Formation for Its Own Secretion To Increase Cell Proliferation and Migration." in: mBio, Vol. 11, Issue 3, (2020) (PubMed).

Rayaprolu, Gao, Xiao, Ramesha, Weinstock, Shah, Duong, Dammer, Webster, Lah, Wood, Betarbet, Levey, Seyfried, Rangaraju: "Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer's disease." in: Molecular neurodegeneration, Vol. 15, Issue 1, pp. 28, (2020) (PubMed).

Lee, Hanchate, Kondoh, Tong, Kuang, Spray, Ye, Buck: "A psychological stressor conveyed by appetite-linked neurons." in: Science advances, Vol. 6, Issue 12, pp. eaay5366, (2020) (PubMed).

Chen, Mohammad, Pazdernik, Huang, Bowman, Tycksen, Schedl: "GLP-1 Notch-LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1." in: PLoS genetics, Vol. 16, Issue 3, pp. e1008650, (2020) (PubMed).

Li, Bao, Luo, Yoan, Sullivan, Quintanilla, Wickersham, Lazarus, Shin, Song: "Supramammillary nucleus synchronizes with dentate gyrus to regulate spatial memory retrieval through glutamate release." in: eLife, Vol. 9, (2020) (PubMed).

Schäfer, Kaisler, Scheller, Kirchhoff, Haghikia, Faissner: "Conditional Deletion of LRP1 Leads to Progressive Loss of Recombined NG2-Expressing Oligodendrocyte Precursor Cells in a Novel Mouse Model." in: Cells, Vol. 8, Issue 12, (2020) (PubMed).

Lehner, Spitzer, Gehwolf, Wagner, Weissenbacher, Deininger, Emmanuel, Wichlas, Tempfer, Traweger: "Tenophages: a novel macrophage-like tendon cell population expressing CX3CL1 and CX3CR1." in: Disease models & mechanisms, Vol. 12, Issue 12, (2020) (PubMed).

Ganjam, Bolte, Matschke, Neitemeier, Dolga, Höllerhage, Höglinger, Adamczyk, Decher, Oertel, Culmsee: "Mitochondrial damage by α-synuclein causes cell death in human dopaminergic neurons." in: Cell death & disease, Vol. 10, Issue 11, pp. 865, (2020) (PubMed).

Bongartz, Gille, Hessenkemper, Mandel, Lewitzky, Feller, Schaper: "The multi-site docking protein Grb2-associated binder 1 (Gab1) enhances interleukin-6-induced MAPK-pathway activation in an SHP2-, Grb2-, and time-dependent manner." in: Cell communication and signaling : CCS, Vol. 17, Issue 1, pp. 135, (2020) (PubMed).

Javier-Torrent, Marco, Rocandio, Pons-Vizcarra, Janes, Lackmann, Egea, Saura: "Presenilin/γ-secretase-dependent EphA3 processing mediates axon elongation through non-muscle myosin IIA." in: eLife, Vol. 8, (2020) (PubMed).

Shibata, Iseda, Mitsuhashi, Oka, Shindo, Moritoki, Nagai, Otsubo, Inoue, Sasaki, Akazawa, Takahashi, Schalek, Lichtman, Okano: "Large-Area Fluorescence and Electron Microscopic Correlative Imaging With Multibeam Scanning Electron Microscopy." in: Frontiers in neural circuits, Vol. 13, pp. 29, (2020) (PubMed).

Turnham, Smith, Kenerson, Omar, Golkowski, Garcia, Bauer, Lau, Sullivan, Langeberg, Ong, Riehle, Yeung, Scott: "An acquired scaffolding function of the DNAJ-PKAc fusion contributes to oncogenic signaling in fibrolamellar carcinoma." in: eLife, Vol. 8, (2020) (PubMed).

Zibetti, Liu, Wan, Qian, Blackshaw: "Epigenomic profiling of retinal progenitors reveals LHX2 is required for developmental regulation of open chromatin." in: Communications biology, Vol. 2, pp. 142, (2020) (PubMed).

Kockel, Griffin, Ahmed, Fidelak, Rajan, Gould, Haigney, Ralston, Tercek, Galligani, Rao, Huq, Bhargava, Dooner, Lemmerman, Malusa, Nguyen, Chung, Gregory, Kuwana, Regenold, Wei, Ashton, Dickinson et al.: "An Interscholastic Network To Generate LexA Enhancer Trap Lines in Drosophila. ..." in: G3 (Bethesda, Md.), Vol. 9, Issue 7, pp. 2097-2106, (2020) (PubMed).

van der Helm, Barnhoorn, de Jonge-Muller, Molendijk, Hawinkels, Coenraad, van Hoek, Verspaget: "Local but not systemic administration of mesenchymal stromal cells ameliorates fibrogenesis in regenerating livers." in: Journal of cellular and molecular medicine, Vol. 23, Issue 9, pp. 6238-6250, (2020) (PubMed).

Crowther, Lim, Asrican, Albright, Wooten, Yeh, Bao, Cerri, Hu, Ian Shih, Asokan, Song: "An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus." in: Stem cell reports, Vol. 10, Issue 3, pp. 1146-1159, (2019) (PubMed).

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抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

背景

Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.

UniProt

P42212