AGO5 抗体
This 兔 多克隆 antibody specifically detects AGO5 in IF, IP 和 WB. It exhibits reactivity toward Arabidopsis thaliana.
产品编号 ABIN1720795
发货至:
中国
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Quick Overview for AGO5 抗体 (ABIN1720795)
抗原
AGO5
(Argonaute Family Protein (AGO5))
适用
Arabidopsis thaliana
宿主
兔
克隆类型
多克隆
应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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原理
- Anti-AGO5 | Argonaute 5
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特异性
- TAIR: At2g27880
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交叉反应 （详细）
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Arabidopsis thaliana
Not reactive in: Hordeum vulgare, Populus sp., Solanum lycopersicum, Zea mays -
预测反应
- Arabidopsis thaliana
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated peptide derived from Arabidopsis thaliana AGO5 protein sequence
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应用备注
- 1 : 100 (IF), 5 μg per gram floral tissue (IP), 1 : 1500 (WB)
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说明
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AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- AGO5 (Argonaute Family Protein (AGO5))
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别名
- AGO5
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背景
- AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). Probably involved in antiviral RNA silencing.
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分子量
- 111 | 111 kDa
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基因ID
- 817334
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UniProt
- Q9SJK3
抗原
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