SARS-CoV-2 Spike S1 protein-coupled magnetic beads
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- 抗原 See all SARS-CoV-2 Spike S1 products
- SARS-CoV-2 Spike S1
- 适用
- SARS Coronavirus-2 (SARS-CoV-2)
- 应用范围
- Flow Cytometry (FACS), Stimulation (St)
- 原理
- SARS-CoV-2 Spike S1 protein-coupled magnetic beads
- 产品特性
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The pre-coupled magnetic beads coupled with biotinylated SARS-CoV-2 Spike S1 protein to streptavidin conjugated magnetic beads, which can capture the Anti- SARS-CoV-2 antibody or ACE2 protein from cell or serum sample.
The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results.
This very first SARS-CoV-2 Spike protein S1-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready to use products could greatly save your time and hassle. - 过滤
- 0.22 μm filtered
- 组件
- Coupled amount of S1 protein: 50 μg / 2 mg beads
- Affinity
- Capture ability of Antibody/Sample: > 200 nmol / mg Beads
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- 应用备注
- This product is intended for immunocapture, cell stimulating, biopanning and flow cytometry.
- 说明
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- Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.
- Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.
- Cell stimulating: In order to study some downstream mechanisms, this antigen coupled beads can be used to stimulate your target cells. It can be easily removed using a magnet when its work is done.
- Biopanning: Biopanning is an affinity selection technique which selects for peptides that binds to a given target. In the capturing step, just add the antigen couple beads to your phage library. Simply use a magnet to separate the bound phages from the unbound ones. More efficient and time saving compared to the plate based capture.
- Flow cytometric analysis: This antigen coupled beads can be used as feeder cells (antigen-presenting cells) to run your flow cytometric analysis, but without the hassle of cell culture.
- 实验流程
- The Antigen pre-Coupled magnetic beads are Coupled with Biotinylated protein onto streptavidin (SA) magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 Mol/L, the Biotinylated protein can bind to the SA beads irreversibly. We provide the SARS-CoV-2 Spike S1 protein-coupled magnetic beads, which could help you to capture the antibody or ACE-2 protein, and easily to follow up with other tests, such as Cell stimulating, biopanning or Flow cytometric analysis.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Ultrapure water
- 缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS, 0.05 % Tween-20, pH 7.4, with 10 % Trehalose
- 注意事项
- Do not to freeze thaw the Beads after reconstitution.
- 储存条件
- -20 °C
- 储存方法
- Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state. Once the Beads reconstitution, please use it immediately.
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- 抗原
- SARS-CoV-2 Spike S1
- Abstract
- SARS-CoV-2 Spike S1 产品
- 别名
- E2 Accessory Reagents, Surface Glycoprotein Accessory Reagents, S Accessory Reagents
- 物质类
- Viral Protein
- 基因ID
- 43740568
- UniProt
- P0DTC2
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