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- 抗原 See all CD8 products
- CD8
- 适用
- 人
- 品牌
- BD IMag™
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- 说明
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BD IMag™ Human CD8 T Lymphocyte Enrichment Set-DM
- 限制
- 仅限研究用
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- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Antibody or streptavidin was conjugated to the magnetic particles under optimum conditions, and unconjugated antibody/streptavidin was removed. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. 557941 Rev. 1 Page 1 of 3 Enrichment of CD8 T lymphocytes from human blood. PBMC were labeled with the BD™ IMag Human CD8 T Lymphocyte Enrichment Set - DM and separated on the BD™ IMagnet (Cat. No. 552311) according to the accompanying Protocol. To demonstrate the efficiency of the enrichment, cells were stained with APC-conjugated anti-human CD8 mAb RPA-T8 (Cat. No. 555369) to detect CD8 T lymphocytes and a mixture of PE-conjugated RPA-T4 (Cat. No. 555347), ICRF44 (Cat. No. 555388), 3G8 (Cat. No. 555407), HIB19 (Cat. No. 555413), CB38 (NL07) (Cat. No. 555455), B159 (Cat. No. 555516), 7G3 (Cat. No. 554529), GA-R2 (HIR2) (Cat. No. 555570), and B1 (Cat. No. 555717) monoclonal antibodies to detect non-CD8 T leukocytes and erythrocytes. Dead cells were excluded by staining with propidium iodide. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
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- 抗原
- CD8
- Abstract
- CD8 产品
- 别名
- CD8 Accessory Reagents, Leu2 Accessory Reagents, MAL Accessory Reagents, p32 Accessory Reagents, CD8a molecule Accessory Reagents, CD8A Accessory Reagents
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