In mice, CD4+ T cells can be divided into naive and memory cells based on expression of adhesion molecules CD44 and CD62L. Naive T cells exhibit high levels of CD62L and low expression of CD44, whereas memory T cells are identified by high CD44 and low CD62L expression. This panel includes antibodies against CD3, CD4, CD62L and CD44, which can be used together to identify naive and memory T cell subsets in mice. Alterations in these subsets are evident in older mice following antigenic stimulation.
组件
Mouse Naive/Memory T cell ID Panel, Rat IgG2b/Rat IgG2a/Rat IgG2a/Rat IgG2b
This reagent contains one vial each of APC/Cy7 conjugated anti-CD3 (clone 17A2), PerCP/Cy5.5 conjugated anti-CD4 (clone RM4-5), APC conjugated anti-CD62L (clone MEL-14), and PE conjugated anti-CD44 (clone IM7) at optimal concentration for four-color flow cytometric analysis.