小鼠 TrueBlot® Anti-小鼠 Ig Biotin

Biotin Mouse TrueBlot® ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Biotin Mouse TrueBlot® ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Biotin Mouse TrueBlot® ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Note: There are two key procedural considerations:
1. When using any TrueBlot® reagent, ensure there is complete reduction of the lysate.
2. Use milk powder for complete and effective blocking of the western blot.

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: Proprietary