(15 references)
-
- 适用
- 小鼠
- 宿主
- 山羊
- 克隆类型
- 多克隆
- 标记
- Agarose Beads
- 应用范围
- Immunoprecipitation (IP), Western Blotting (WB)
- Supplier Product No.
- 00-8811-25
- Supplier
- Rockland
- 品牌
- TrueBlot®
- 产品特性
-
TrueBlot® anti-Mouse Ig IP Beads are a suspension of activated agarose beads coupled with goat anti-mouse IgG. It is suitable for precipitation of mouse IgGs used as the primary antibodies in immunoprecipitation assays. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Conjugation Name: Agarose beads for TrueBlot® - 组件
- TrueBlot® Anti-Mouse Ig IP Beads
-
-
- 应用备注
-
Immunoprecipitation Dilution: TrueBlot® anti-Mouse Ig IP Beads (binds 0.4 mg Ig/mL beads) have been reported for use in IP
Western Blot Dilution: Use with Mouse TrueBlot® (ABIN1589977) - 说明
-
Upon initial use of this product, we recommend that the vial be inverted several times to get the beads into suspension. We recommend to use a large bore pipet to pipet up the liquid for use. For storage of the opened vial, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer.
- 实验流程
-
Preparation of Immunoprecipitated Sample for SDS-PAGE:
1. Preclear cell lysate: Add 50 µL of Anti-Mouse IgG Beads and 500µl of cell lysate sample to a microcentrifuge tube and incubate on ice for
3. minutes. Spin at 10,000xg for 3 minutes and transfer the supernatant to a new microcentrifuge tube.
2. Immunoprecipitation: Add 5 µg of primary antibody to the microcentrifuge tube containing the precleared lysate. Incubate on ice for 1 hour. Add 50 µL of Anti-Mouse IgG Beads. Incubate for 1 hour on a rocking platform. Spin the microcentrifuge tube at 10000xg for 1 minute. Remove supernatant completely and wash the (pelleted) beads 3 times with 500 µL of Lysis Buffer (50mM Tris HCl pH 8.0, 150mM NaCl,1 % NP-40).
3. Prepare sample for SDS-PAGE: After the last wash, aspirate supernatant, and add 100 µL Laemmli Buffer (with 50 mM DTT or 1 %ß-mercaptoethanol, final) to bead pellet. Vortex and heat to 90-100 °C for
1. minutes. Spin at 10000xg for 3 minutes, collect supernatant, and load onto the gel. Avoid loading Anti-Mouse Ig Beads.
Note: The supernatant can be stored at -20 °C for future use. After thawing, add fresh dithiothreitol and heat as above. Centrifuge the sample at 10000xg for 1 minute in a microcentrifuge tube to pellet any Anti-Mouse Ig Beads and immediately transfer an aliquot of the supernatant to gel wells. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
-
Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium AzideStabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Do not freeze.
Sensitive to light.
Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume. - 储存条件
- 4 °C
- 储存方法
- Store vial at 4 °C prior to opening.
- 有效期
- 6 months
-
-
: "IL-34 and protein-tyrosine phosphatase receptor type-zeta-dependent mechanisms limit arthritis in mice." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 102, Issue 8, pp. 846-858, (2022) (PubMed).
: "L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis." in: Cell reports, Vol. 38, Issue 11, pp. 110507, (2022) (PubMed).
: "GAGE mediates radio resistance in cervical cancers via the regulation of chromatin accessibility." in: Cell reports, Vol. 36, Issue 9, pp. 109621, (2022) (PubMed).
: "MLL5 is involved in retinal photoreceptor maturation through facilitating CRX-mediated photoreceptor gene transactivation." in: iScience, Vol. 25, Issue 4, pp. 104058, (2022) (PubMed).
: "A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis." in: Nature communications, Vol. 12, Issue 1, pp. 7333, (2022) (PubMed).
: "Control of cell migration by the novel protein phosphatase-2A interacting protein inka2." in: Cell and tissue research, Vol. 380, Issue 3, pp. 527-537, (2021) (PubMed).
: "Unique Immune Cell Coactivators Specify Locus Control Region Function and Cell Stage." in: Molecular cell, Vol. 80, Issue 5, pp. 845-861.e10, (2020) (PubMed).
: "Androgen receptor activation reduces the endothelial cell proliferation through activating the cSrc/AKT/p38/ERK/NFκB-mediated pathway." in: The Journal of steroid biochemistry and molecular biology, Vol. 194, pp. 105459, (2020) (PubMed).
: "NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma." in: Oncogene, Vol. 36, Issue 27, pp. 3820-3830, (2017) (PubMed).
: "Co-regulation of mitochondrial respiration by proline dehydrogenase/oxidase and succinate." in: Amino acids, Vol. 48, Issue 3, pp. 859-872, (2016) (PubMed).
: "MLL5 maintains spindle bipolarity by preventing aberrant cytosolic aggregation of PLK1." in: The Journal of cell biology, Vol. 212, Issue 7, pp. 829-43, (2016) (PubMed).
: "Distinct pathways regulate Syk protein activation downstream of immune tyrosine activation motif (ITAM) and hemITAM receptors in platelets." in: The Journal of biological chemistry, Vol. 290, Issue 18, pp. 11557-68, (2015) (PubMed).
: "The coactivator role of histone deacetylase 3 in IL-1-signaling involves deacetylation of p65 NF-κB." in: Nucleic acids research, Vol. 41, Issue 1, pp. 90-109, (2013) (PubMed).
: "Identification and functional characterization of novel phosphorylation sites in TAK1-binding protein (TAB) 1." in: PloS one, Vol. 6, Issue 12, pp. e29256, (2012) (PubMed).
: "c-Jun N-terminal kinase phosphorylates DCP1a to control formation of P bodies." in: The Journal of cell biology, Vol. 194, Issue 4, pp. 581-96, (2011) (PubMed).
-
: "IL-34 and protein-tyrosine phosphatase receptor type-zeta-dependent mechanisms limit arthritis in mice." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 102, Issue 8, pp. 846-858, (2022) (PubMed).
-
You are here:
