GM-CSF Protein (AA 8-144)
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- 抗原 See all GM-CSF (CSF2) 蛋白
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 8-144
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宿主
- 人
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资源
- Tobacco (Nicotiana benthamiana)
- 应用范围
- Western Blotting (WB), Cell Culture (CC)
- 序列
- HHHHHHHHHH APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
- 特异性
- Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific GM-CSF antibody.
- 产品特性
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Molecular Formula: C699H1077N201O206S8
Isoelectric Point: 6,21
Extinction Coefficient: E 0.1 % (1g/L) = 0.898 (A 280 nm) - 内毒素水平
- < 0.04 EU/µg protein (LAL method)
- Top Product
- Discover our top product CSF2 蛋白
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein
CSF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 8-144) proteinCSF2 宿主: 人 宿主: Tobacco (Nicotiana benthamiana) Recombinant WB, CC
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein (His tag)CSF2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) proteinCSF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein (Fc Tag)CSF2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) proteinCSF2 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) protein (His tag)CSF2 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-144) protein (GST tag)CSF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) (Active) protein (His tag)CSF2 宿主: 人 宿主: HEK-293 Cells > 95 % CC, AcA Active
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- 说明
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rHuman GM-CSF is a glycosylated polypeptide chain containing 127 amino acids (18-144 aa CSF2_HUMAN P04141 ), fused to 10 His tag at N-terminal. rHuman GM-CSF migrate as a broad band between 15 and 25 kDa due to post-translation modification, in particular glycosylation. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Granulocyte-macrophage colony-stimulating factor (GM-CSF) contains a 10-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal-derived components or impurities. Animal free product. The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with coomassie blue.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Lyophilized protein should be reconstituted in water to a concentration of 25-50 ng /µL. Optimal concentration should be determined for specific application and cell lines.
- 缓冲液
- 10 mM PBS buffer pH 7.6 and 0.2 M NaCl
- 储存条件
- 4 °C
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- 抗原
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- 别名
- GM-CSF (Granulocyte-macrophage colony-stimulating factor ) (CSF2 产品)
- 别名
- GMCSF Protein, GM-CSF Protein, CSF2 Protein, Csfgm Protein, Gm-CSf Protein, MGI-IGM Protein, Gm-csf Protein, Gmcsf Protein, CSF Protein, colony stimulating factor 2 Protein, colony stimulating factor 2 (granulocyte-macrophage) Protein, CSF2 Protein, Csf2 Protein
- 背景
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Synonyms: Granulocyte-macrophage colony-stimulating factor (GM-CSF), Colony-stimulating factor (CSF), Molgramostin, Sargramostim
GMCSF is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. It´s involved in differentiation of dendritic cells and is a key factor in differentiation pathways leading form stem cells. GMCSF is produced by several cell types as monocytes, fibroblasts, endothelial cells and T- Lymphocytes in response to a number of inflammatory mediators present in the hemopoietic environment and peripheral site of inflammation. Human GMCF is an important therapeutic cytokine used in the treatment of myeloid leukemia, neutropenia and aplastic anemia and it could become interesting in the treatment following bone marrow transplantation. It performs its biological activity by binding to a specific receptor complex which is composed of a cytokine-specific alpha chain and beta chain, shared with the receptors for interleukin-3 and interleukin-5. GMCSR has been identified to mediate the activation of Jak-Stat and MAPK pathways. - UniProt
- P04141
- 途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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